Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)
Identifieur interne : 000073 ( France/Analysis ); précédent : 000072; suivant : 000074Characterization of blood mononuclear cells producing IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)
Auteurs : B. Charley [France] ; L. Lavenant [France]Source :
- Research in Immunology [ 0923-2494 ] ; 1990.
English descriptors
- Teeft :
- Alpha interferon, Antiviral activity, Ascitic fluids, Cell monolayers, Cell surface glycoproteins, Cellules, Cellules infect6es, Coronavirus, Dendritic cells, Dengue virus, Direct effect, Final dilution, Gastroenteritis, Glycoprotein, Herpes, Herpes simplex virus, Human lymphocytes, Ifn0t, Ifn0t induction process, Ifn0t production, Ifna, Ifna production, Igg2a, Induction, Influenza virus, Interferon, Interferon production, Kidney cells, Laude, Lebon, Lesser extent, Leukocyte, Lymphocyte, Major histocompatibility, Membrane interactions, Membrane structures, Monoclonal, Monoclonal antibodies, Monoclonal antibodies reactive, Monolayers, Msa3, Msa3 hybridoma culture, Mycoplasma membranes, Other antisla class, Overnight incubation, Pbmc, Pbmc responsiveness, Porcine, Porcine cells, Pretreatment, Producer cells, Rabbit serum, Sendai virus, Surface antigens, Tgev, Transmissible gastroenteritis coronavirus, Transmissible gastroenteritis virus, Viable cells, Viral glycoprotein, Virus particles.
Abstract
Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.
Url:
DOI: 10.1016/0923-2494(90)90133-J
Affiliations:
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ISTEX:44EE75741B0741123E24817D35B40C8FF2F998E0Le document en format XML
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Charley</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et d'Immunologie moléculaires, INRA</wicri:regionArea><wicri:noRegion>INRA</wicri:noRegion><wicri:noRegion>INRA</wicri:noRegion></affiliation></author><author><name sortKey="Lavenant, L" sort="Lavenant, L" uniqKey="Lavenant L" first="L." last="Lavenant">L. Lavenant</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et d'Immunologie moléculaires, INRA</wicri:regionArea><wicri:noRegion>INRA</wicri:noRegion><wicri:noRegion>INRA</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Research in Immunology</title><title level="j" type="abbrev">X83</title><idno type="ISSN">0923-2494</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">141</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="141">141</biblScope><biblScope unit="page" to="151">151</biblScope></imprint><idno type="ISSN">0923-2494</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0923-2494</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Alpha interferon</term><term>Antiviral activity</term><term>Ascitic fluids</term><term>Cell monolayers</term><term>Cell surface glycoproteins</term><term>Cellules</term><term>Cellules infect6es</term><term>Coronavirus</term><term>Dendritic cells</term><term>Dengue virus</term><term>Direct effect</term><term>Final dilution</term><term>Gastroenteritis</term><term>Glycoprotein</term><term>Herpes</term><term>Herpes simplex virus</term><term>Human lymphocytes</term><term>Ifn0t</term><term>Ifn0t induction process</term><term>Ifn0t production</term><term>Ifna</term><term>Ifna production</term><term>Igg2a</term><term>Induction</term><term>Influenza virus</term><term>Interferon</term><term>Interferon production</term><term>Kidney cells</term><term>Laude</term><term>Lebon</term><term>Lesser extent</term><term>Leukocyte</term><term>Lymphocyte</term><term>Major histocompatibility</term><term>Membrane interactions</term><term>Membrane structures</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibodies reactive</term><term>Monolayers</term><term>Msa3</term><term>Msa3 hybridoma culture</term><term>Mycoplasma membranes</term><term>Other antisla class</term><term>Overnight incubation</term><term>Pbmc</term><term>Pbmc responsiveness</term><term>Porcine</term><term>Porcine cells</term><term>Pretreatment</term><term>Producer cells</term><term>Rabbit serum</term><term>Sendai virus</term><term>Surface antigens</term><term>Tgev</term><term>Transmissible gastroenteritis coronavirus</term><term>Transmissible gastroenteritis virus</term><term>Viable cells</term><term>Viral glycoprotein</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Porcine blood mononuclear cells (PBMC) were shown to produce interferon-α (IFNα) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFNα producer cells. The production of IFNα was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Charley, B" sort="Charley, B" uniqKey="Charley B" first="B." last="Charley">B. Charley</name></noRegion><name sortKey="Lavenant, L" sort="Lavenant, L" uniqKey="Lavenant L" first="L." last="Lavenant">L. Lavenant</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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